FreezeM https://www.freezem.com Mon, 16 Dec 2024 17:54:48 +0000 en-US hourly 1 https://wordpress.org/?v=6.7.1 https://www.freezem.com/wp-content/uploads/2024/02/cropped-logotipo-FreezeM-32x32.pngFreezeMhttps://www.freezem.com 32 32 2024 in Review for FreezeM and the BSF Industryhttps://www.freezem.com/2024-in-review-for-freezem-and-the-bsf-industry/ Mon, 16 Dec 2024 17:06:39 +0000 https://www.freezem.com/?p=2360

The Black Soldier Fly (BSF) industry has had an extraordinary year in 2024, marked by growth, innovation, and a shift toward more sustainable and efficient practices. Companies have refined their approaches, focusing on specialization and scalable solutions, while insect-based proteins have gained broader acceptance, particularly in pet food and aquaculture.

FreezeM has been at the forefront of these changes, scaling operations, forming transformative partnerships, and driving technological advancements reshaping the industry. Reflecting on the past year, we celebrate our achievements and look forward to the exciting opportunities ahead in 2025.

Production Capacities Are Increasing

According to GlobeNewswire, the BSF market is projected to reach $3.96 billion by 2033, growing at a compound annual growth rate (CAGR) of 31% from 2024 to 2033. In terms of volume, the market is expected to reach 8.23 million tons by 2033, with a CAGR of 40.4% during the same period.

This expansion is driven by rising global meat demand, a growing aquaculture industry, and increased interest from the animal feed sector seeking sustainable protein alternatives.

Functional Added-Value Products

Research into BSF has unveiled its potential beyond traditional applications. BSF larvae efficiently convert organic waste into high-protein biomass, offering sustainable solutions for animal feed and biofertilizers. Innovations include BSF-derived chitin and chitosan, which have applications in agriculture and pharmaceuticals. Some companies are exploring using BSF larvae as bioreactors to produce biotechnological products such as growth factors and enzymes.

FreezeM is also taking part in the exploration of these exciting directions and has generated a state-of-the-art genetic platform for BSF genetic modification.

Insect Protein Gains Acceptance in Pet Food and Aquaculture

Insect-based proteins have gained traction in the pet food industry, and they are recognized for their hypoallergenic properties and sustainability. The pet food sector is expected to become the second-largest consumer of insect protein by 2030, with a projected demand of 150,000 metric tons.

At the same time, in aquaculture, insect protein is increasingly viewed as a sustainable alternative to fishmeal, with successful trials demonstrating its efficacy in fish diets. Once production capacity and target price reach a critical inflection point, the adoption of insect meal in aquaculture will accelerate, creating exciting growth opportunities for the industry.

Strategic Corporate Realignments: Focused and Specialized Approaches
Companies within the BSF industry are adopting more pragmatic strategies, emphasizing specialization and focus. This shift involves pursuing less capital-intensive projects with medium-scale capacities, fostering sustainable growth. Increasingly, companies are concentrating on either rearing or processing while outsourcing the complex, resource-intensive breeding.

Hybrid models—where in-house breeding is supplemented with outsourced production—or fully outsourced agreements are becoming more prevalent.

The Pivotal Role of Waste Management

Effective waste management has become integral to the BSF industry. BSF larvae can convert organic waste into valuable protein, oil, and biofertilizers, positioning them as a sustainable solution for waste reduction. This capability is the base of the initiatives taking place between BSF producers and waste management companies, aiming to address environmental challenges while creating economic value.

FreezeM’s Development in 2024

Scaling Up Production and the Partnership with Hermetia

In 2024, FreezeM took a bold step forward in scaling up its operations through an innovative partnership with Hermetia. Together, we established the first pan-European BSF breeding hub strategically located in Germany. This facility not only serves as a center of excellence for BSF breeding but also exports globally, marking a milestone in the industry’s ability to supply high-quality neonates on an international scale. By centralizing production and distribution, this hub has set a benchmark for efficiency and global reach, catering to the rising demand for insect-based solutions. The breeding hub produces our core product, PauseM, at an industrial scale, and it already serves BSF-rearing partners throughout Europe in various models – from complete decoupled breeding to hybrid supply.

Commercializing and Proving Our Model at Scale

This year also saw the commercialization of FreezeM’s Breeding-as-a-Service model at an unprecedented scale. Partnering with Agroloop, which has established the world’s first fully outsourced BSF breeding facility. This collaboration introduced a groundbreaking innovation to the industry: the automated PauseM deployment machine.
This state-of-the-art technology is a game-changer, enabling industrial-scale BSF production by automating the insertion of up to 4,000 PauseM units—equivalent to 200 million neonate larvae—into production lines daily. This efficiency not only streamlines operations but also reduces labor costs, positioning FreezeM and Agroloop at the forefront of BSF industry innovation.

Learn more about the Agroloop partnership >>

Transforming Waste Management: The Schachar Collaboration

In partnership with Schachar Group, FreezeM is redefining waste management using Black Soldier Fly technology. Schachar, known for its expertise in recycling industrial and commercial food waste, has joined forces with FreezeM to create a fully integrated solution. Schachar’s subsidiary, Binge Organics, formulates specialized BSF diets from food waste, while FreezeM enhances the production process through its Breeding-as-a-Service model. Together, we are converting wet food waste streams into valuable animal feed and biofertilizers, delivering both environmental benefits and economic growth. This collaboration highlights the crucial role of BSF technology in sustainable waste management.

Strengthening the Israeli BSF Ecosystem

2024 also marked the second period of the Israeli BSF consortium, funded by the Israeli Innovation Authority (IIA), which FreezeM is proud to be part of. With renewed focus and collaboration, the consortium has made promising strides in advancing BSF research and industry practices within Israel. These efforts set the stage for innovative developments in breeding and genetics techniques, novel protein and oil production methods, new applications in animal feed and agriculture, regulatory engagement, and most importantly, creating a novel AI-based model for the generation of optimized BSF rearing recipes based on variable and seasonal waste streams, further solidifying Israel’s role as a critical player in the global BSF landscape, and setting the technological infrastructure for facing future challenges.

R&D Spearhead

FreezeM is not left behind regarding biotechnological applications of BSF and has developed an advanced platform for the precise gene editing of the black soldier fly genome, setting the ground for developing new strains with enhanced features (like the previously reported BSF-Titan). We have also made breakthrough progress in the development of OSDel-BSF technology for directed delivery of genetic materials to the BSf female ovary, a project we are working on with the Isam Khalaila lab from Ben Gurion University. Throughout the year, we have also developed a molecular kit for high throughput male/female classification of BSF via PCR.

What’s next for 2025?

Scaling Operations in Europe and Israel

In 2025, FreezeM plans to enhance its production capacities across Europe and Israel, capitalizing on the growing demand for sustainable protein sources. By leveraging our established partnerships and innovative technologies, we aim to increase output and efficiency, solidify our position as a Black Soldier Fly (BSF) industry leader, and take another step towards implementing our decoupled production model, enabling industry growth.

Expanding into the United Kingdom Market

The United Kingdom presents a promising market for BSF products, driven by a solid commitment to sustainable agriculture and increasing acceptance of insect-based proteins. The UK government has shown support for alternative proteins, aligning with global trends toward sustainable food sources. FreezeM intends to enter the UK market by collaborating with local partners and adhering to regulatory standards to meet the rising demand for eco-friendly protein alternatives.

New product improvements

As we continue refining our products based on valuable client feedback, we look forward to achieving significant milestones in 2025. Our plans include extending PauseM’s shelf life, introducing a variety of packaging options tailored to diverse production settings, and offering innovative complementary tools designed to enhance the implementation and value creation across a range of BSF farming scenarios and scales.

Conclusion

As 2024 draws to a close, the Black Soldier Fly industry is at a pivotal juncture characterized by remarkable growth, innovation, and a shifting focus toward specialization and sustainability.

FreezeM is proud to have contributed to these advancements, scaling production, forging transformative partnerships, and introducing groundbreaking technologies.
Looking ahead to 2025, we remain committed to expanding our impact. We will scale up operations in Europe and Israel, enter promising markets like the UK, and navigate new regulatory landscapes that promise to unlock fresh opportunities. Together, we’re not just advancing our company but pushing the boundaries of what’s possible in sustainable agriculture and waste management. Stay with us as we continue to lead the way in creating a more sustainable, circular future.

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BSF Eggs. The Foundation of Every Successful Black Soldier Fly Farmhttps://www.freezem.com/bsf-eggs-the-foundation-of-every-successful-black-soldier-fly-farm/ Tue, 24 Sep 2024 13:35:28 +0000 https://www.freezem.com/?p=2214

In recent years, Black Soldier Fly (BSF, Hermetia Illucens) farming has gained significant attention as a sustainable and profitable solution for waste management and high-quality protein production. At the heart of this process are BSF eggs, the foundation of every successful BSF colony. This article aims to provide an in-depth understanding of BSF eggs, from identification and collection to the intricacies of breeding and hatching. Whether you are an established BSF farmer or someone exploring this exciting venture, this guide will offer you valuable insights.

BSF. It all starts with an egg

BSF eggs are the starting point of a highly efficient natural cycle. Once hatched, BSF larvae can consume large amounts of organic waste, converting it into valuable by-products, such as frass (organic fertilizer) and high-protein larvae, which are ideal for animal feed. For farmers and businesses, this process presents a sustainable way to manage waste while generating new revenue streams. The quality of BSF eggs directly impacts the yield and efficiency of the entire process, making egg management a critical aspect of insect farming.

The natural BSF cycle

A female Black Soldier Fly typically lays eggs only once in her lifetime. After mating, she will lay a single cluster of eggs, usually in secluded areas near a food source but not directly on it. Each female can lay between 600 to 900 eggs during this process, releasing them one by one using a specialized organ through a process known as oviposition. Once the eggs are laid, the female fly dies shortly after, as her reproductive role is complete. The life cycle of the Black Soldier Fly focuses on producing a large number of offspring in a single laying event to maximize reproductive success.

The weight of a single BSF egg is approximately 30 micrograms. Initially, the eggs are white but gradually turn yellow as they mature. In the final stages of their development, close observation under a microscope reveals the development of the larvae’s eyes, indicating they are nearly ready to hatch. It’s important to note that the egg itself is the embryo, and the female fly fertilizes the eggs as they pass through the oviposition tube, utilizing stored male sperm from the mating process.

Large-Scale Oviposition

In large-scale BSF breeding operations, guiding females to lay eggs in the desired locations presents a considerable challenge. BSF females tend to deposit their eggs in various unintended spots within the breeding environment, such as walls, corners, or even dead flies, leading to significant egg loss—sometimes reducing yield by as much as several dozen percent.

In large-scale BSF breeding operations, guiding females to lay eggs in the desired locations presents a considerable challenge.

While improvised attractants are often used to influence where females lay eggs, these attractants are frequently homemade, using methods like fermentation or other biological processes. Unfortunately, these solutions tend to be inconsistent and unstable, particularly when scaled up, which can lead to further egg yield losses. Natural fluctuations in egg production further complicate matters, necessitating overproduction—often 1.5 to 3 times the actual requirement—to ensure a sufficient supply of eggs for rearing.

Furthermore, the sensitivity of BSF eggs means that improper incubation can lead to poor hatch rates and inconsistent timing. Once hatched, neonates must be promptly introduced to feed at the correct larvae-to-feed ratio to optimize their growth and ensure efficient feed conversion (FCR). Mismanagement of oviposition, hatching, and neonate dosing can significantly reduce breeding efficiency, negatively impacting overall production performance.

How to Collect BSF Eggs Efficiently

Efficient egg collection is essential for maintaining a healthy BSF colony. It’s crucial to check the traps regularly, as the eggs need to be collected within a few days to ensure proper hatching conditions. Once collected, the eggs should be carefully transferred to a controlled environment, where temperature and humidity can be optimized to encourage hatching.

When collecting eggs, it’s crucial to maintain cleanliness to avoid contamination. A sterile environment helps prevent the growth of mold or other pathogens that could jeopardize egg fertility or the health of the larvae.

How Long Do BSF Eggs Take to Hatch?

BSF eggs typically take about 3 to 4 days to hatch, but this timeline can vary depending on environmental factors. Temperature is a critical factor: the ideal range for hatching is between 26°C to 30°C. Humidity also plays a vital role, with optimal conditions being around 70%. Deviations from these conditions can delay hatching or reduce the viability of the larvae. Farmers should regularly monitor these factors and ensure a stable environment to maximize hatch rates.

Best Attractants for BSF Egg Laying

BSF flies are naturally attracted to decomposing organic matter, which serves as a food source for their larvae. However, certain attractants can be used to maximize egg-laying rates. Well-prepared waste piles that emit strong odors are often the most effective. Common attractants include fruit and vegetable waste, particularly those with high moisture content, such as bananas, melons, and other decaying produce. It is important to strike a balance—while waste attracts the flies, placing the eggs directly on moist waste can negatively impact hatching. Thus, offering dry, secluded spots near the waste is essential.

Challenges in Breeding BSF

While insect farming offers significant advantages, successfully breeding black soldier flies can be complex. Maintaining the right environmental conditions, ensuring a consistent food supply, and managing the reproduction cycle are common challenges farmers face. Exposure to these conditions can lead to a decrease in overall colony performance, affecting not just larvae quality, but also fertility and survival.

Additionally, the breeding process requires specialized knowledge and equipment to ensure optimal outcomes. Many farmers find it difficult to maintain genetic diversity and pathogen control within their colonies, which can lead to reduced larvae quality over time.

Black Soldier Fly (Hermetia illucens) Embryonic Development Stages

The FreezeM Advantage: Simplifying BSF Egg Management

This is where FreezeM comes in. At FreezeM, we understand the complexities involved in BSF breeding, and we offer a streamlined solution for insect farmers. Our breeding solutions allow farmers to outsource the reproduction process so they can focus on rearing and production. FreezeM handles the complexities of large-scale breeding, from egg-laying to hatching, and provides neonates in a live-suspension state, ready to be used whenever your waste feed is prepared. This method ensures that you receive healthy, robust larvae exactly when you need them, without worrying about the timing or the intricate details of hatching.

By outsourcing your breeding needs to FreezeM, you can maintain consistent production without investing heavily in breeding infrastructure. Additionally, this approach ensures that your colony maintains genetic diversity and pathogen control, which are critical to long-term success.

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Black Soldier fly embryonic development - by FreezeM nonadult
Breeding Black Soldier Fly Larvae In-House. Top 5 Challenges and Pitfallshttps://www.freezem.com/breeding-black-soldier-fly-larvae-in-house-top-5-challenges-and-pitfalls/ Mon, 19 Aug 2024 09:28:29 +0000 https://www.freezem.com/?p=2172

Insect farming, particularly Black Soldier Fly (Hermetia Illucens) farming, is gaining traction as a sustainable protein production and waste management solution. However, managing Black Soldier Fly breeding (reproduction) and maintaining an in-house Black Soldier Fly colony present several challenges that can hinder efficiency and scalability.

This article explores the top challenges Black Soldier Fly (BSF) farmers face. It highlights the benefits of an outsourced or hybrid solution, where buying BSF neonates can mitigate many of these issues.

Challenge 1. Production Stability and Predictability

 

Breeding Consistency

Maintaining breeding consistency is crucial for the success of BSF farming. Consistency ensures larvae develop uniformly, leading to predictable yields and high-quality protein production. However, achieving this consistency takes time and effort.

Accurate Dosing and Feed Amounts

Accurate dosing and feed amounts are critical to maintaining consistency in BSF farming. Overfeeding or underfeeding can result in suboptimal growth conditions, affecting the larvae’s health and development. Monitoring and adjusting feed levels require meticulous attention and expertise, which can be challenging to maintain consistently in-house.

Neonate Quality

The quality of neonates, or newly hatched larvae, significantly impacts the overall stability of the production. In-house breeding requires precise control over breeding conditions to produce high-quality, synchronized neonates in a consistent manner. Variability in neonate quality can lead to uneven growth rates and reduced yields, complicating the farming process. Furthermore, it can also lead to a suboptimal feed conversion rate (FCR) and, subsequently, to increased feed expenses.

Live-Suspended Neonates with PauseM® Technology

FreezeM’s flagship product, PauseM®, provides live-suspended BSF larvae neonates that can be halted for up to 14 days. Each unit contains a synchronized and pre-counted number of neonates, ensuring high consistency and reproducibility between trays. This technology optimizes feed inputs and enhances feed consumption efficiency, addressing the production stability challenge head-on.

Learn more about PauseM® >

Challenge 2. Breeding and Egg Production

 

Fly Colony Management

Maintaining a healthy fly colony is crucial for continuous egg production. However, managing the colony can be challenging due to the specific requirements for emerging, mating and egg-laying (oviposition). Flies need optimal conditions to mate successfully, including appropriate lighting, temperature, and humidity. Any disruption in these conditions can reduce mating success and egg production.

Risk of Disease Outbreaks

Like any form of animal husbandry, insect farming is susceptible to disease outbreaks. Infections can spread rapidly in dense populations, leading to significant losses. Maintaining biosecurity in in-house BSF farming operations involves quantitative and quality control to prevent contamination and manage diseases, which can be labor-intensive and costly. In addition, BSF facilities attract other fly species that can contaminate the colony, reducing performance and may lead to safety issues.

Egg Harvesting and Handling

Another significant challenge in BSF breeding is collecting and handling eggs efficiently. Eggs are delicate and require careful handling to prevent damage. Ensuring eggs are collected, incubated, and hatched under optimal conditions is essential for producing neonates with high fitness. This process is labor-intensive and requires precise timing and techniques to maximize hatch rates and neonate quality.

Challenge 3. Genetic Drifts

 

Inbreeding Depression

A lack of genetic diversity due to repeated inbreeding within a limited population can lead to genetic drifts. This results in weakened individuals with reduced fertility, slower growth rates, and higher susceptibility to diseases.

Accumulation of Mutations

Harmful mutations can accumulate in a closed population over generations. Without introducing new genetic material, these mutations can have a cumulative negative effect on the colony’s overall health and productivity.

Adaptation to Suboptimal Conditions

A closed breeding environment can lead to the population adapting to the specific conditions of the facility, which may not be ideal for optimal growth and reproduction. This narrow adaptation can reduce the population’s ability to thrive in different environments or conditions, making the colony less adaptable to changes or stressors.

The Hybrid Breeding Model

Outsourcing part of the Black Soldier Fly (BSF) breeding process enhances genetic diversity. It mitigates the risks of inbreeding depression by introducing flies with diverse genetic backgrounds to refresh the colony. Regular genetic refreshment through outsourcing keeps the colony dynamic and adaptable, preventing stagnation and maintaining long-term viability. Leveraging specialized breeding facilities’ expertise and advanced techniques like FreezeM’s ensures optimal breeding practices and precise genetic management, resulting in a healthier, more productive, and resilient colony.

Learn more about FreezeM’s BSF Technology>

Challenge 4. Technical and Operational Complexity

 

Growth Conditions

Optimal growth conditions, including temperature, humidity, and light, are essential for efficient BSF breeding. Any deviations from the ideal conditions can dramatically affect the reproduction performance of a colony. Maintaining these conditions consistently requires advanced technology and continuous monitoring, adding to the operational complexity.

Advanced Farming Systems

Setting up and maintaining an efficient BSF farming operation requires advanced farming systems, including automated feeding and climate control systems. These technologies are essential for optimizing production but come with high initial costs and require specialized knowledge to operate and maintain.

Labor and Expertise

BSF breeding demands a skilled workforce knowledgeable in entomology and biology, as well as dedicated labs and breeding facilities. Recruiting and training staff to meet these requirements can be challenging, especially in regions where insect farming is not widely practiced. The operational complexity increases as the production scale grows, necessitating a larger and more skilled workforce.

Challenge 5. Scaling Up

The transition from small-scale to large-scale BSF breeding involves overcoming significant challenges related to equipment, environmental control, work protocols, biological behavior, and manpower. Effectively addressing these issues is crucial for the sustainable growth and success of BSF breeding facilities.

Implementing New Equipment

Implementing new equipment is often costly and requires extensive operator training. These new systems also demand numerous dry runs and meticulous calibration, a process that many companies find more challenging than initially anticipated. While reliable rearing and processing systems are available on the market and can be acquired from several suppliers, this is not the case for breeding. In this case, breeding capex is more risky and can result in a higher depreciation rate due to frequent upgrades or installments of new systems.

Stabilizing Growth Conditions

Maintaining optimal conditions for BSF growth is crucial, but in large rooms, this task becomes far from trivial. Inconsistent conditions can profoundly affect growth rates and escalating operational costs, making precise environmental control a critical component of successful scale-up.

Revising Work Protocols

Work protocols that function well on a small scale often need adaptation for larger operations. Many tasks that were previously performed manually must be automated to maintain efficiency. However, suitable automation solutions are not always readily available and may require custom development and adjustment. This transition to automation necessitates further workforce training to ensure smooth operation.

Adapting to Biological Changes

Biological factors also come into play, as insects tend to behave differently when reared in large quantities. This means biological setups effective at a small scale may need to be re-established or modified for larger-scale operations. Understanding and adapting to these behavioral changes are essential to maintaining colony health and productivity.

Advantages of Outsourced and Hybrid Breeding Models

Outsourced or hybrid breeding solutions offer significant advantages for BSF farming operations by addressing critical challenges associated with in-house breeding. One of the key benefits is the separation of breeding and rearing, which highlights the importance of the specialized knowledge and investment required for successful breeding. By outsourcing breeding or adopting a hybrid model, facilities can ensure a consistent quality and supply of neonates, which is crucial for maintaining a stable and productive process.

This approach streamlines operations and reduces the complexities and costs associated with breeding, allowing farmers to focus on growing larvae, improving productivity, and scaling their operations faster and more efficiently. By purchasing high-quality neonates from specialized suppliers, farmers can leverage the expertise and resources of professional breeding facilities, ensuring the long-term health and viability of their BSF colonies.

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Why Waste Management Companies Should Invest in Insect Farminghttps://www.freezem.com/why-waste-management-companies-should-invest-in-insect-farming/ Mon, 29 Jul 2024 06:49:14 +0000 https://www.freezem.com/?p=2139

As the world grapples with the dual challenges of food security and environmental sustainability, innovative solutions are emerging at the intersection of agriculture and waste management. One such promising solution is insect farming.

Insect farming’s circular approach tackles the pressing issue of food waste while providing a renewable source of protein, contributing to a more resilient and environmentally friendly feed system.

For waste management companies, venturing into insect farming not only represents a lucrative business opportunity but also a sustainable approach to waste utilization and food production. Several waste management companies are already at the forefront of the industry, utilizing black soldier fly (BSF) technology to address food waste sustainably.

Veolia, for example, is a global industry leader harnessing the power of BSF larvae to transform organic waste from agricultural and agri-food sectors into high-protein animal feed. Their initiatives in Malaysia and France not only help manage waste but also contribute to sustainable food production by providing an alternative protein source while reducing the environmental footprint of traditional livestock farming​.

Similarly, GoTerra, in Australia, has introduced modular waste management units, known as Modular Infrastructure for Biological Services (MIBs), that utilize BSF larvae to process food waste onsite. Their project at the Barangaroo Towers in Sydney demonstrates the efficacy of this approach in converting high-volume food waste into insect protein and soil enhancers, significantly reducing emissions and supporting the circular economy.​​

Transforming Waste into Value

Waste management companies deal with vast amounts of organic waste, which often ends up burned or in landfills, contributing to greenhouse gas emissions and environmental degradation. Insect farming, particularly the cultivation of Black Soldier Flies (BSF), offers a compelling alternative. BSF larvae can consume a wide variety of organic waste, transforming it into high-value protein and oil. This process not only reduces the volume of waste but also creates new revenue streams.

Key Benefits:

Waste Reduction: BSF larvae can process twice their body weight in organic waste daily, significantly reducing the volume of waste.

High-Value Products: The larvae are rich in protein (up to 45%) and fat (up to 35%), making them an excellent ingredient for animal feed, including aquaculture, poultry, and pet food.

Economic Viability

According to Rabobank, the global market for insect protein will reach $9.46 billion by 2030, driven by increasing demand for sustainable animal feed. Waste management companies are uniquely positioned to capitalize on this growing market. By integrating insect farming into their operations, they can diversify their revenue streams and enhance their profitability.

Economic Drivers:

Growing Demand: The aquaculture and pet food industries are actively seeking sustainable protein alternatives to fishmeal and soybean meal, both of which have significant environmental impacts.

Cost Efficiency: Utilizing organic waste as feed for insects reduces raw material costs, improving the economic viability of insect farming.

Environmental Sustainability

Insect farming is a highly sustainable practice with a minimal environmental footprint. It addresses several critical environmental issues, including waste management, greenhouse gas emissions, and land use.

Environmental Impact:

-Efficient Resource Use: Insects require less land and water than conventional livestock or vegetal protein, making them a sustainable protein source.

Circular Economy: By converting waste into valuable organic products, insect farming supports the principles of a circular economy, reducing reliance on finite resources.

Addressing Specific Challenges with Wet Organic Waste

Wet organic waste poses significant challenges for traditional waste management processes. It has a shorter shelf life and higher water content, making transportation and handling less economically efficient.

These waste streams are often directed to biogas production, which, while offering renewable energy benefits, misses the potential for higher-value upcycling.

Biogas also comes with significant challenges. The high initial costs for setting up biogas plants can be prohibitive, especially for small-scale operations. Maintaining the optimal conditions for anaerobic digestion, such as temperature and pH, is challenging and can affect efficiency. Moreover, if not managed properly, biogas production can cause environmental pollution from effluents and even methane leakage.

BSF Farming as a Solution:

BSF larvae thrive on wet organic waste, which typically comprises 60-70% moisture. This makes BSF farming an ideal solution for managing wet waste streams effectively. The collaboration between FreezeM and Shachar Group demonstrates how this model can be successfully implemented. FreezeM’s decoupled model, which supplies ready-to-use BSF neonates, allows waste management companies to convert wet waste into valuable insect protein without the need for on-site breeding facilities.

A case study about waste managent companies converting  waste into animal feed

Strategic Synergies and Case Study Insights

The collaboration between FreezeM and Shachar Group exemplifies the benefits of integrating insect farming into waste management operations. Shachar, specializing in upcycling food waste, faced challenges with wet waste streams. By leveraging FreezeM’s BSF technology, they transformed wet waste into valuable resources.

Case Study Highlights:

– Scalability and Efficiency: FreezeM’s PauseM® BSF neonates enable scalable and efficient insect farming operations by outsourcing the breeding process and focusing on their production capabilities.

– Innovative Solutions: Shachar’s pilot rearing facility explores various waste streams, optimizing BSF feed recipes and achieving superior feed conversion rates.

– Environmental and Economic Impact: This collaboration not only reduces waste but also generates new revenue streams through the production of high-value protein and organic fertilizer.

Conclusion

Insect farming represents a transformative opportunity for waste management companies. By turning organic waste into high-value protein and oil, companies can enhance their profitability, contribute to environmental sustainability, and support the growing demand for sustainable animal feed. With regulatory support, technological advancements, and strategic synergies, waste management companies are well-positioned to lead the way in this innovative and sustainable industry.

As the world seeks sustainable solutions to pressing environmental and food security challenges, insect farming offers a promising path forward. Waste management companies that embrace this opportunity can play a crucial role in shaping a more sustainable and resilient future.

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Using CRISPR to Improve Insect Protein for Sustainable Feedhttps://www.freezem.com/using-crispr-to-improve-insect-protein/ Wed, 14 Feb 2024 06:25:00 +0000 https://www.freezem.com/?p=547

In the dynamic realm of sustainable food production, a remarkable transformation is underway, led by a pioneering group of companies championing insect larvae as a revolutionary protein source. FreezeM has been recognized among these trailblazers, leveraging cutting-edge CRISPR technologies to elevate the efficiency and nutritional quality of black soldier fly (BSF) larvae.

Breaking Ground with CRISPR Technology

CRISPR, or Clustered Regularly Interspaced Short Palindromic Repeats, is a revolutionary gene-editing technology that has significantly impacted various fields, including agriculture and biotechnology. CRISPR allows scientists to precisely modify the DNA of organisms, including, of course, insects, by targeting specific genes.

Genetic Enhancement: CRISPR enables the targeted enhancement of specific traits in insects, aligning with the goals of insect-based animal feed production. This could involve modifying genes related to growth, reproduction, or resistance to environmental stressors.

Adaptation to Production Scales: By using CRISPR, we can potentially tailor the genetic makeup of insects to adapt seamlessly to diverse production setups and volumes, providing scalability to meet the global demand for sustainable animal feed.

Environmental Sustainability: CRISPR offers the opportunity to create insect lines that are more environmentally sustainable. This includes traits that enhance resource utilization, enable the upcycling of more waste streams using BSF, reduce ecological impact, and contribute to the overall efficiency of insect farming as a sustainable alternative in animal feed production.

 

Enhancing BSF Strains with Precision Gene Editing

Our commitment to pushing boundaries at FreezeM led us to harness the power of CRISPR–Cas9 technology, resulting in the enhancement of BSF strains. Deploying 400 guide RNAs targeting approximately 150 genes controlling metabolism, we’ve achieved strains with larger larvae, and prolonged larval periods. Notably, our gene-editing efforts include a focus on the sema-1a ortholog, a key regulator influencing molting. The result? Larvae that are a staggering 50% larger than their wild counterparts.

The versatility of our genetic engineering platform enables us to capitalize on the promising research path of reducing chitin in insect exoskeletons, a breakthrough that holds great promise for enhancing aquaculture feed efficiency and further solidifying our commitment to tailoring BSF strains for specific applications.

BSF Titan: 50% Bigger

FreezeM is developing advanced genetic lines of black soldier flies (BSF), leveraging CRISPR gene-editing for the optimization of growth, reproduction, and environmental resistance traits.

The innovation includes the BSF-Titan, a groundbreaking development in BSF genetic engineering. The BSF-Titan line phenotype is a 50% bigger larvae that maintains the same nutrition values (protein, oil ect.) as the wild type strain.

 

BSF-Titan utilizes CRISPR-Cas9 gene-editing methodologies to enhance BSF growth  and protein content.

This line, in its final development stages, aims to enhance size and protein content, showcasing a 50% increase in larvae size and improved protein production yield. The BSF-Titan line could be obtained through traditional methods, complying with European regulatory non-GMO standards. BSF Titan marks a significant stride in sustainable and scalable insect farming for animal nutrition.

 

Ensuring safe and ethical genetic engineering practices

Achieving safe and ethical genetic engineering requires careful consideration of the underlying molecular mechanisms and the potential downstream effects of genetic modifications. It is crucial to ensure that any genetic modifications comply with established ethical guidelines and that the resulting organisms remain safe and viable for their intended applications. The work of our experienced scientific team at FreezeM acknowledges a comprehensive understanding of the genetic pathways involved, as well as the potential risks and benefits associated with genetic engineering.

 

Empowering the Future with Genetic Engineering

As we look forward to the future, enhanced genetic lines, like FreezeM’s first demonstration of BSF-Titan, are expected to open new horizons and applications for the BSF farming industry, and enable it to fulfill its potential.

FreezeM’s cooperation with the Khalaila lab at Ben-Gurion University of the Negev pushes the boundaries of CRISPR-Cas9 editing in Black Soldier Flies (BSF). Unlike the conventional method limited to editing one egg per injection, FreezeM, alongside Dr. Khalaila’s lab, pioneers a revolutionary approach enabling the simultaneous editing of hundreds of eggs in a single injection. This groundbreaking process involves a complex comprising CRISPR machinery and OSDel, a peptide facilitating delivery into developing female oocytes.

Targeting adult females allows editing of the DNA of all progenies, opening avenues for large-scale CRISPR editing with potential heritable changes across generations. Generously funded by the Israel Innovation Authority, this project underscores FreezeM’s commitment to advancing biotechnological innovations in BSF genetics. The genetic engineering platform promises superior BSF lines tailored to specific applications, amplifying production efficiency and overall performance.

 

The Larger Context and Future Prospects

These revolutionary methods align with the broader context of a burgeoning insect protein market, projected to reach $1.14 billion by 2027. Our contributions to the consortium, supported by the Israel Innovation Authority, exemplify collaborative efforts aimed at optimizing and scaling up BSF production. As the global demand for sustainable protein sources intensifies, FreezeM’s innovations represent a significant stride toward establishing insect larvae as a viable and eco-friendly alternative.

In conclusion, FreezeM’s pioneering work in utilizing CRISPR technologies to enhance insect protein for feed underscores the transformative potential of genetic engineering in sustainable agriculture. As we continue to lead the way in developing efficient and scalable solutions, the broader insect farming industry stands to benefit from advancements that could reshape the future of animal feed production.

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Unveiling the Enigmatic World of the BSF Larvaehttps://www.freezem.com/unveiling-the-enigmatic-world-of-the-bsf-larvae/ https://www.freezem.com/unveiling-the-enigmatic-world-of-the-bsf-larvae/#respond Thu, 01 Feb 2024 15:23:35 +0000 https://www.freezem.com/?p=887

In the realm of insects, the Black Soldier Fly (BSF) and its larvae have emerged as fascinating subjects of study, capturing the attention of researchers and environmentalists alike. BSF transcends mere entomology. It offers a glimpse into the complex tapestry of nature’s recycling systems. It is a tropical fly species with exceptional organic matter decomposition capabilities and a myriad of applications ranging from protein-rich animal and fish feed to the production of valuable byproducts like oleochemicals and frass. These enigmatic larvae are proving to be ecological game-changers.

 

The Black Soldier Fly and Its Extraordinary Lifecycle

The Black Soldier Fly, scientifically known as Hermetia illucens, is an insect native to Latin America, but it has expanded to almost every region on the planet. Thriving in temperatures between 25 to 35 degrees Celsius, its broad temperature tolerance makes it adaptable to diverse environments.

What makes these creatures particularly intriguing is their unique life cycle. The adult BSF does not consume solid food and has a lifespan of only a few days, during which its primary goal is to reproduce. The female lays eggs, and once hatched, the larvae become the focus of attention due to their incredible transformative abilities. Larvae undergo a staggering 8,000-fold size increase in a mere 14 days.

To put it in perspective, this rapid growth is like a human baby transforming into a full-sized adult gray whale weighing over
20,000 kg in just a couple of weeks!

Lyfe Cycle

Egg Stage: The life cycle begins with the egg stage, where female black soldier flies lay their eggs in cracks and crevices near decaying organic matter, such as compost or food waste. The female black soldier fly deposits a mass of 200 to 900 eggs at a time, which hatch into larvae in about four days. Each oval-shaped egg is about 1 mm in length.

Larva Stage: The larva stage is where the real action happens. The larvae can reach up to 300mg after six moltings (insters), during which they shed their exoskeleton to accommodate their growing size. They are a dull, whitish color with a small, projecting head containing chewing mouthparts. Larvae require approximately 14 days to complete development. These larvae are voracious eaters with a high turnover rate for the feed, capable of consuming large amounts of organic material in a short time. They play a critical role in breaking down and recycling waste, transforming it into nutrient-rich frass and biomass.

Pupa Stage: During this stage, the larvae stop feeding and search for a place to pupate (pre-pupa). Their walking in line in search of a place to pupate is why the fly got its name – Black Soldier Fly. They enter the pupa stage, where they undergo metamorphosis to transform into adult black soldier flies. Pupation requires about two weeks. This is a non-feeding stage focused on development.

Adult Stage: Adult black soldier flies do not consume solid food. They have a wasp-like appearance and are black or blue in color. They also have two translucent “windows” located on the abdomen. Adults may range from 15 to 20 mm in length and their primary focus is on mating and reproducing, completing the life cycle. They are non-pest insects, ie, beneficial to our ecosystem, and do not cause damage to property or crops, pose health risks or are a nuisance as other insects.

A Striking Appearance

Cloaked in a mysterious blend of white and black hues, BSF (Black Soldier Fly) don a distinctive conical shape optimized for interacting with organic matter. Delicate hairs grace their bodies, lending them an almost otherworldly appearance. Despite their unassuming demeanor, these insects undergo remarkable growth, reaching an impressive size of up to 1 inch (2.5 cm) during their larval stage.

Physical Characteristics

Color: Black Soldier Fly neonates have white tones, but as they grow, larvae are typically dark brown to black. This coloration makes them easy to spot, especially in compost or waste material.

Conical Shape: These larvae have a distinctive conical shape, with a wider posterior end and a tapering, pointed head. This shape aids them in burrowing into their food source.

Size: Black Soldier Fly larvae grow to an impressive length of around 1 inch (2.5 cm) during their larval stage, making them one of the larger fly larvae species.

Fine Hairs: Their bodies are covered in tiny, fine hairs, which may help them move through their environment and interact with their surroundings.

 

Efficient Waste Converters

These unique characteristics and life stages make Black Soldier Fly larvae valuable contributors to waste management, sustainable agriculture, and various ecological processes. Their efficiency as decomposers and nutrient-rich composition make them an eco-friendly solution to several environmental and food security challenges.

In a mere two weeks, 500 grams of neonate larvae have the astounding ability to devour up to 15 tonnes of waste, generating an impressive one ton of protein in the process.

BSF larvae are highly efficient in converting organic waste into biomass. They can consume a wide range of organic materials. Additionally, they display resilience against microorganisms and parasites, surviving in challenging environments while effectively cooperating with microorganisms to degrade organic waste.

Regulations, though, prohibit feeding certain materials to insects, especially those classified as meat, manure or urban waste. These regulations are very strict on what are permitted insect substrates. These may include vegetal matter, such as cereal grains, legume seeds, roots, fruits, forages and algae, and non‑animal-containing industry by‑products from bakery and confectionery industries and from processing fruit and vegetables. Certain animal by-products as substrates are also permitted, including milk, cooked eggs, and fats…provided that the substrates have undergone processing such as pasteurizing, cooking or baking under the hygiene regulations.

 

BSF Larvae As a Protein Factory

Dried and frozen Black Soldier Fly larvae can be used in animal feed applications directly or further processed into products such as BSF meal powder, puree and BSF oil.
One of the most significant contributions of BSF larvae lies in their protein content. Studies have shown that these larvae are exceptionally rich in protein and other nutrients, including essential amino acids, unsaturated fatty acids, minerals, and vitamins, making them an ideal candidate for animal and fish feed.

The nutrient amounts largely depend on the quality of food, with about 18–33% fat and 32–53% protein.

With a protein content in the range of traditional feed sources like soy (40-48%) and fish meal (60-68%), BSF larvae present a sustainable alternative that can help address global concerns about the environmental impact of traditional feed production, such as land -and sea resources- exhaustion, water consumption or greenhouse gas emissions.

Research indicates that BSF larvae and BSF meal have excellent anti-inflammatory, antimicrobial, and hypoallergenic properties, resulting in health improvements in the animals that consume them. One of the main applications is in the production of hypoallergenic foods for pets.

A Circular Solution to Organic Waste

Beyond their role in the food chain, BSF larvae contribute to a sustainable and circular economy. As the larvae consume organic waste, they not only reduce the environmental burden of waste disposal but also create valuable byproducts such as oil and frass.

Insect oil is obtained by the process of defatting insect proteins, is highly digestible and provides a sustainable source of energy for many animals. The lipid content of BSF larvae/prepupae is influenced by the feed for growth and can reach more than 50%.

BSF lipids mainly consist of medium-chain saturated fatty acids, with lauric acid (LA) being the dominant component. LA has been reported to exhibit antibacterial, antifungal, antiviral, and anticancer properties. Moreover, it has applications in various fields, such as pharmaceuticals, cosmetics, body care, plastics, and textiles.

The excrement of the larvae, known as frass, is a nutrient-rich fertilizer that contains essential nutrients like nitrogen, phosphorus, and potassium, contributing to soil enrichment and plant growth. However, many aspects related to frass are still unknown, such as its varying composition of nutrients, microorganisms and bioactive compounds, its behavior in the soil and its action in the plants’ metabolism, among other aspects that are subject to numerous studies.

Research Insights: Unlocking the Potential

Scientific research on BSF larvae continues to unveil new possibilities and applications. Studies have explored optimal conditions for larvae growth, refining techniques for mass production, development of strains to increase protein or oil yield, and assessing the nutritional composition of the larvae. The results are promising, pointing towards a future where BSF larvae play a crucial role in sustainable agriculture, waste management, and alternative protein production.

BSF-Titan® is a genetically engineered BSF line developed by FreezeM that aims to enhance its size and protein content through CRISPR-Cas9 gene-editing methodologies. BSF-Titan® is the first Black Soldier Fly line showcasing augmented production performance, exhibiting a 50% increase in larvae size and significantly improving protein production yield.

To learn more about FreezeM technology >>

Conclusion: Paving the Way for a Sustainable Future

In nature’s grand tapestry, Black Soldier Fly larvae are unsung heroes, weaving a story of transformation as they turn organic waste into valuable resources. Their applications range from protein-rich feeds for livestock and aquaculture to eco-friendly fertilizers and industrial chemicals, showcasing the vast potential of these remarkable insects.

As we unravel the mysteries of their world, we discover not only ecological solutions but also a path toward a more sustainable and harmonious future. The multifaceted benefits of Black Soldier Fly larvae serve as a testament to the beauty of nature’s intricate design and its capacity to address the challenges of our time.

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A Year of Milestones: FreezeM’s 2023 Recap and Future Aspirationshttps://www.freezem.com/freezem-2023-recap-and-future-aspirations/ https://www.freezem.com/freezem-2023-recap-and-future-aspirations/#respond Mon, 15 Jan 2024 09:08:38 +0000 https://www.freezem.com/?p=1

As we bid farewell to 2023, allow me a moment to reflect on a year filled with accomplishments, growth, and innovation. The entire team achieved remarkable milestones in our growth as a market leader in the insect industry, and I want to take this opportunity to guide you through some of the key highlights that have defined our year.

In a year marked by significant growth in the Black Soldier Fly (BSF) industry, FreezeM has played a pivotal role, setting new standards and contributing to the industry’s advancement. Join me as we explore these milestones and the exciting path ahead for FreezeM within the flourishing landscape of the BSF industry.

 

1. Expanding Horizons: Launching Production in Europe with Hermetia Collaboration

In a groundbreaking move, FreezeM extended its reach by establishing a collaboration with Hermetia GmbH, one of the pioneers of BSF farming, marking our production launch in Europe. This strategic partnership broadens our geographical footprint and strengthens our commitment to sustainable and efficient Black Soldier Fly breeding practices. We can now support customers throughout Europe at scale from one centralized breeding hub.

 

2. Product Evolution: Advancements in PauseM®

Driven by customer feedback and a relentless pursuit of excellence, FreezeM continued to enhance PauseM®, our flagship product. This year saw significant improvements in product-market fit, increased compatibility with automation systems, and optimization for large-scale supply operations. These developments underscore our dedication to providing a seamless and efficient outsourcing solution for BSF breeding. During the last year, we have sent PauseM® to various locations worldwide and tested its performance in different production settings – from large-scale centralized operations to pilot facilities or decentralized, modular rearing units. The insights we gained were priceless, demonstrating the superior performance on various feedstocks and growth settings.

 

3. Genetic Breakthrough: Introducing BSF Titan and GFP-BSF

A momentous achievement for FreezeM in 2023 was the successful generation of the first enhanced BSF genetic line, BSF Titan, as covered in Feed&Additive magazine, leveraging our advanced genetics platform. BSF Titan larvae can reach a 50% higher weight with an improved feed conversion rate (FCR), demonstrating the potential of using targeted genetics to enhance larvae performance. This groundbreaking accomplishment showcases our commitment to pushing the boundaries of innovation in insect breeding. Additionally, FreezeM proudly presents the world’s first GFP-BSF, demonstrating our leadership in genetic research within the insect-based protein production industry.

4. Industry Recognition: Embracing the Decoupled Production Model

FreezeM’s visionary approach to separate reproduction (breeding) and production (rearing and processing) has gained widespread recognition throughout the industry. More players are adopting innovative breeding solutions, including outsourcing and hybrid (combined outsource and in-house breeding) models, positioning FreezeM at the forefront of pioneering practices that redefine the future of BSF breeding. More and more BSF industry newcomers can now see they can enter the field with much lower investment and grow faster by adopting our decoupled model.

 

5. Team Growth: Welcoming New Talent and Celebrating Company Culture

The FreezeM family welcomed new and extraordinary talent in 2023, further enriching our collective vision and goals. In the face of challenging times, these remarkable individuals have demonstrated tremendous resilience, grit, and a shared determination that unites us as a team. Their strength and commitment have become a beacon of inspiration.
To showcase the vibrant culture that fuels our success, here’s a glimpse of the latest company fun day held in Tel Aviv. Meet the faces behind FreezeM and witness the collaborative spirit that drives our achievements.

 

6. Continued Innovation: Leading in R&D Excellence

FreezeM remains dedicated to pushing the boundaries of innovation through ongoing research and development initiatives. Two notable projects, the BSF-Israel consortium launch and collaboration with the Khalaila lab at  BGN, exemplify our commitment to advancing BSF breeding technologies. As we delve into new frontiers like genetics, FreezeM reaffirms its position as an innovation and R&D excellence leader, as stated by Nature Biotechnology magazine in its coverage of CRISPR advancements and Insect Tech.

 

Looking Ahead: Building on Momentum in 2023 and Embracing Change

As we step into the future, FreezeM is poised to build on the momentum of 2023. Our focus remains on growth, technological advancement, and staying at the forefront of BSF breeding innovation, making it simple for new players to enter the market. However, the excitement doesn’t stop there. We are working on a complete rebranding in response to our remarkable growth and maturity. It’s time to reflect on our evolution in a fresh, innovative way that truly captures the essence of our journey. Stay tuned as we continue to revolutionize the industry and unveil soon a brand-new identity that mirrors our commitment to excellence and sustainability.

In 2024, we plan to expand our production in Europe and Israel, creating more exciting new technologies and collaborations to enhance insect protein production globally and enable it to fulfill its potential.

Thank you for being an integral part of our story. Here’s to the memories we’ve made, the challenges we’ve overcome, and the exciting journey ahead.

With gratitude,
Yuval Gilad, CEO, FreezeM

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